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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Nature. 2021 Apr 7;592(7855):611–615. doi: 10.1038/s41586-021-03440-3

Extended Data Figure 7. Type III-A CRISPR-Cas immunity against ϕStaph1N phage.

Extended Data Figure 7.

(a) Serial ten-fold dilutions of ϕStaph1N phage were spotted on plates seeded with different strains of staphylococci expressing a gp14-targeting S. epidermidis type III-A system with different mutations, wild-type or (Ind-) LexA, or the S. aureus type II-A system. (b) PCR amplification of the CRISPR arrays of the wild-type pCRISPR plasmid isolated from 16 individual colonies resistant to ϕStaph1N phage that were previously exposed to or not treated with ϕNM1γ6 phage. Molecular markers (in kilobases) are shown on the left; the PCR product of the non-expanded CRISPR array is marked by the arrowhead.