Table 1.
Messenger RNA
| Cell Line | VPAC1-R | VPAC2-R | PAC1-R | VIP | PACAP |
|---|---|---|---|---|---|
| SCLC | |||||
| H209 | + | − | + | + | + |
| H345 | − | + | + | + | + |
| N417 | + | − | + | + | + |
| H510 | + | − | + | − | + |
| NSCLC | |||||
| H157 | + | − | − | + | − |
| H727 | + | − | + | + | − |
| H838 | + | − | + | + | + |
| H1264 | − | + | + | − | + |
Note: Complementary DNA made from the mRNA by reverse transcriptase. The cDNA was added to TAQ polymerase and the following primers were used. VPAC1-R primers were 5′-ATGTGCAGATGATCGAGGTG-3′ (sense) and 5′-TGTAGCCGGTCTTCACAGAA-3′ (antisense). The VPAC2-R primers were 5′-CTTCAGGAAGCTGCACTGC-3′ (sense) and 5′-CAAACACCATGTAGTGGACG-3′ (antisense). The PAC1-R primers were 5′CATCCTTGTGCAGAAACTTC-3′ (sense) and 5′GGTGCTTGAAGTCCACAGCG-3′ (antisense). PCR was performed at 94°C for 45 sec, 62°C for 30 sec, and 72°C for 45 sec, using 40 cycles.