Skip to main content
. Author manuscript; available in PMC: 2022 Feb 7.
Published in final edited form as: Cell Rep. 2020 Dec 8;33(10):108489. doi: 10.1016/j.celrep.2020.108489

Figure 2. Serotonergic xbp-1s Is Sufficient to Drive Chaperone Induction and ER Stress Resistance.

Figure 2.

(A) Fluorescent micrographs of day 1 adult hsp-4p::GFP control or three independent integration lines of serotonergic xbp-1s (tph-1p::xbp-1s) animals. All images are contrast matched. CE, contract enhanced images for clarity; some pixels may be saturated. Only the lower 60% of the worm is shown to remove co-injection marker signal.

(B) Quantification of (A): day 1 adult hsp-4p::GFP control (gray) or serotonergic xbp-1s (tph-1p::xbp-1s; green) animals. Lines represent median and interquartile range, with each dot representing a single animal. Data are representative of three independent trials. ***p < 0.001 using non-parametric Mann-Whitney testing against control. Quantification is applied in the lower 60% of the worm to remove co-injection marker signal.

(C) Fluorescent micrographs of day 1 adult hsp-4p::GFP control or serotonergic xbp-1s (tph-1p::xbp-1s; line 3 was selected for all further analyses) animals with and without tph-1(mg280) mutation for serotonin synthesis. Some pixels may be saturated. Only the lower 60% of the worm is shown to remove co-injection marker signal.

(D) Quantification of (C): day 1 adult hsp-4p::GFP control (gray) or serotonergic xbp-1s (tph-1p::xbp-1s; green) animals. Lines represent median and interquartile range, with each dot representing a single animal. Data are representative of three independent trials. ***p < 0.001 using non-parametric Mann-Whitney testing against matching control. Quantification is applied in the lower 60% of the worm to remove co-injection marker signal.

(E) Tunicamycin (TM) survival assay of control and serotonergic xbp-1s (tph-1p::xbp-1s) animals. Animals were moved to 25 ng/μL TM plates at day 1 of adulthood. Data are representative of three independent trials. See Table S1–S2 for lifespan statistics.

(F) TM survival assay of control and serotonergic xbp-1s (tph-1p::xbp-1s) animals carrying tph-1(mg280) mutation for serotonin synthesis. Animals were moved to 25 ng/μL TM plates at day 1 of adulthood. Assays for (E) and (F) were performed simultaneously and can be directly compared. Data are representative of three independent trials. See Table S1–S2 for lifespan statistics.