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. Author manuscript; available in PMC: 2022 Feb 7.
Published in final edited form as: Cell Rep. 2020 Dec 8;33(10):108489. doi: 10.1016/j.celrep.2020.108489

Figure 4. Overexpression of xbp-1s in Dopaminergic Neurons Drives Lipid Depletion to Extend Lifespan.

Figure 4.

(A) Representative fluorescent micrographs of intestinal lipid droplets (LDs) (by dhs-3p::DHS-3::GFP) in day 2 adult control and dopaminergic xbp-1s (dat-1p::xbp-1s) animals with and without cat-2(n4547) mutation in dopamine synthesis. Imaging was performed equidistant from the vulva to the tail of the worm across all conditions. All images are contrast matched. Scale bar represents 10 μm.

(B) Quantification of dhs-3p::DHS-3::GFP in (a) of control (gray) and dopaminergic xbp-1s (dat-1p::xbp-1s; orange) animals with and without cat-2(n4547) mutation in dopamine synthesis. Lines represent median and interquartile range, with each dot representing a single animal. Data are representative of three independent trials. ***p < 0.001 using non-parametric Mann-Whitney testing against matching control.

(C) Lifespans of control and dopaminergic xbp-1s (dat-1p::xbp-1s) animals grown on empty vector. Data are representative of three independent trials. See Table S1–S2 for lifespan statistics.

(D) Lifespans of control and dopaminergic xbp-1s (dat-1p::xbp-1s) animals grown on ehbp-1 RNAi from hatch. Data are representative of three independent trials. See Table S1–S2 for lifespan statistics.

(E) Lifespan measurements of control and dopaminergic xbp-1s (dat-1p::xbp-1s) animals carrying cat-2(n4547) mutation for dopamine synthesis. Data are representative of three independent trials. See Table S1–S2 for lifespan statistics.

(F) Lifespans of control and dopaminergic xbp-1s (dat-1p::xbp-1s) animals grown on xbp-1 RNAi. Data are representative of three independent trials. See Table S1–S2 for lifespan statistics. Measurements for (D), (E), and (F) were performed simultaneously and can be directly compared.