Table 2.

Effect of CuSO₄ and CuNPs on tissue culture responses of wheat genotypes

A			B			C			D
Treatments			Callus induction (%)			Embryogenic callus (%)			Regeneration (%)
	CuSO4 (mg/L)	CuNPs (mg/L)	AS-2002	Wafaq-2001	Mean	AS-2002	Wafaq-2001	Mean	AS-2002	Wafaq-2001	Mean
C1	0.010	-	79.67	82.67	81.17 g	68.33	62.67	65.50 ef	49.67	50.33	50.00 ef
C2	0.015	-	81.67	82.67	82.17 fg	69.67	70.00	69.83 cd	53.33	51.33	52.33 de
C3	0.020	-	84.67	82.67	83.67 defg	74.00	74.00	74.00 ab	56.00	54.33	55.17 cd
C4	MS based medium with standard CuSO4 0.025 mg/l (Control)		86.00	90.33	88.17 bc	78.33	73.00	75.67 a	59.33	57.00	58.17 bc
C5	0.030	-	92.00	94.33	93.17 a	73.33	68.67	71.00 bcd	49.00	44.33	46.67 f
C6	0.035	-	82.33	91.33	86.83 bcde	67.33	64.67	66.00 ef	38.67	38.33	38.50 g
C7	-	0.010	82.33	84.33	83.33 efg	69.67	67.67	68.67 de	60.00	51.67	55.83 cd
C8	-	0.015	86.67	84.67	85.67 cdef	79.00	74.67	76.83 a	65.67	63.00	64.33 a
C9	-	0.020	87.67	87.67	87.67 bcd	73.00	71.33	72.17 bc	62.67	58.33	60.50 ab
C10	-	0.025	89.33	91.67	90.50 ab	69.33	67.00	68.17 de	51.33	44.00	47.67 f
C11	-	0.030	88.33	92.67	90.50 ab	64.00	62.33	63.17 f	37.33	40.33	38.83 g
C12	-	0.035	82.67	85.67	84.17 cdefg	58.00	53.67	55.83 g	32.33	30.67	31.50 h
Mean			85.64	87.19		70.33 a	67.47 b		51.50 a	48.47 b
			LSD value Genotype NS = 1.6568 Treatments* = 4.0583 Genotype × Treatments NS = 5.7393 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05)			LSD value Genotype * = 1.4184 Treatments* = 3.4745 Genotype × Treatments NS = 4.9136 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05)			LSD value Genotype * = 1.6587 Treatments* = 4.0629 Genotype × Treatments NS = 5.7459 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05)