Table 3.
Effect of AgNO3 and AgNPs on tissue culture responses of wheat genotypes
| A |
B |
C |
D |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Treatments | Callus induction (%) | Embryogenic callus (%) | Regeneration (%) | |||||||||
| CuSO4 (Standard) mg/L |
AgNO3 (mg/L) |
AgNPs (mg/L) |
AS-2002 |
Wafaq-2001 |
Mean |
AS-2002 |
Wafaq-2001 |
Mean |
AS-2002 |
Wafaq-2001 |
Mean |
|
| S1 | MS based medium with standard CuSO4 0.025 mg/l (Control) | 82.33 | 84 | 83.17 fg | 67 | 62.33 | 64.67 f | 56.67 | 55.67 | 56.17 f | ||
| S2 | 0.025 | 1 | - | 81.67 | 83.67 | 82.67 g | 68.33 | 63.33 | 65.83 ef | 57.67 | 57.67 | 57.67ef |
| S3 | 0.025 | 2 | - | 82.67 | 86.00 | 84.33 fg | 67.00 | 67.00 | 67.00 ef | 60.00 | 60.00 | 60.00cde |
| S4 | 0.025 | 3 | - | 83.67 | 88.00 | 85.83 defg | 69.67 | 69.33 | 69.50 de | 62.00 | 61.33 | 61.67bcd |
| S5 | 0.025 | 4 | - | 84.67 | 88.67 | 86.67 cdef | 73.33 | 75.33 | 73.33 cd | 64.33 | 61.00 | 62.67bc |
| S6 | 0.025 | 5 | - | 89.33 | 91.33 | 90.33 abc | 75.00 | 71.67 | 74.33 bc | 65.33 | 64.00 | 64.67ab |
| S7 | 0.025 | 6 | - | 90.333 | 95.67 | 93.00 a | 73.00 | 72.67 | 72.83 cd | 59.33 | 56.67 | 58.00ef |
| S8 | 0.025 | 7 | - | 87.33 | 94.67 | 91.00 ab | 66.67 | 67.00 | 66.83 ef | 47.00 | 42.67 | 44.83 g |
| S9 | 0.025 | - | 1 | 83.33 | 83.33 | 83.33 fg | 68.67 | 65.33 | 67.00 ef | 60.33 | 58.67 | 59.50de |
| S10 | 0.025 | - | 2 | 83.33 | 84.33 | 83.83 fg | 71.00 | 68.33 | 69.67 de | 63.00 | 60.67 | 61.83bcd |
| S11 | 0.025 | - | 3 | 84.67 | 86.67 | 85.67 efg | 77.67 | 77.33 | 77.50 ab | 68.33 | 65.00 | 66.67a |
| S12 | 0.025 | - | 4 | 86.00 | 92.00 | 89.00 bcde | 80.67 | 79.00 | 79.83 a | 61.33 | 63.00 | 62.17bcd |
| S13 | 0.025 | - | 5 | 92.00 | 94.33 | 93.17 a | 72.00 | 72.67 | 72.33 cd | 56.33 | 54.33 | 55.33 f |
| S14 | 0.025 | - | 6 | 89.33 | 90.00 | 89.67 abcd | 69.00 | 64.33 | 66.67 ef | 47.67 | 44.67 | 46.17 g |
| S15 | 0.025 | - | 7 | 85.67 | 87.00 | 86.33 defg | 62.33 | 56.33 | 59.33 g | 41.00 | 37.33 | 39.17 h |
| Mean | 85.76 b | 88.64 a | 70.73 a | 68.82 b | 58.02 a | 56.18 b | ||||||
|
LSD value: Genotype* = 1.4554 Treatments* = 3.9858 Genotype × Treatment = 5.6367 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05) |
LSD value: Genotype* = 1.4479 Treatments* = 3.9652 Genotype × Treatment NS = 5.6077 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05) |
LSD value: Genotype* = 1.1041 Treatments* = 3.0238 Genotype × Treatment NS = 4.2763 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05 |
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