Table 4.
Effect of various combinations of CuNPs and AgNPs on tissue culture responses of wheat genotypes
| A |
B |
C |
D |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Treatments | Callus induction (%) | Embryogenic callus (%) | Regeneration (%) | |||||||||
| |
CuSO4 (mg/l) |
CuNPs (mg/l) |
AgNPs (mg/l) |
AS-2002 |
Wafaq-2001 |
Mean |
AS-2002 |
Wafaq-2001 |
Mean |
AS-2002 |
Wafaq-2001 |
Mean |
| N1 | 0.025 mg/l (Control) | 85.00 | 85.00 | 85.00 defg | 74.67 | 71.67 | 73.17 defg | 59.33 | 56.33 | 57.83 efgh | ||
| N2 | 0.010 | 81.33 | 82.67 | 82.00 g | 71.33 | 67.67 | 69.50 g | 54.33 | 57.67 | 56.00 gh | ||
| N3 | 0.015 | 86.33 | 88.67 | 87.50 bcd | 77.33 | 73.67 | 75.50 bcd | 62.68 | 60.67 | 61.67 cd | ||
| N4 | 0.020 | 83.00 | 83.33 | 83.17 efg | 72.00 | 72.33 | 72.17 defg | 58.67 | 57.67 | 58.17 defgh | ||
| N5 | 0.025 | 2 | 85.67 | 88.67 | 87.17 cde | 75.67 | 74.00 | 74.83 cd | 62.00 | 56.67 | 59.33 cdefg | |
| N6 | 0.025 | 3 | 84.00 | 91.33 | 87.67 bcd | 77.33 | 78.67 | 78.00 abc | 63.67 | 62.00 | 62.83 bc | |
| N7 | 0.025 | 4 | 90.00 | 92.67 | 91.33 ab | 81.68 | 79.33 | 80.50 a | 67.33 | 65.00 | 66.17 b | |
| N8 | 0.010 | 2 | 82.33 | 85.33 | 83.83 defg | 69.33 | 69.00 | 69.17 g | 55.67 | 53.33 | 54.50 h | |
| N9 | 0.010 | 3 | 80.00 | 86.33 | 83.17 efg | 74.00 | 67.00 | 70.50 efg | 58.67 | 53.67 | 56.17 gh | |
| N10 | 0.010 | 4 | 81.00 | 84.67 | 82.83 fg | 71.67 | 68.33 | 70.00 fg | 60.00 | 56.67 | 58.33 defg | |
| N11 | 0.015 | 2 | 90.00 | 91.00 | 90.50 abc | 80.33 | 71.33 | 75.83 bcd | 62.33 | 59.67 | 61.000 cde | |
| N12 | 0.015 | 3 | 84.33 | 90.00 | 87.17 cde | 78.00 | 71.67 | 74.00 cdef | 65.67 | 66.67 | 66.17 b | |
| N13 | 0.015 | 4 | 90.00 | 95.00 | 92.50 a | 84.67 | 78.00 | 80.33 a | 71.67 | 68.33 | 70.00 a | |
| N14 | 0.020 | 2 | 93.67 | 87.33 | 90.50 abc | 77.33 | 81.00 | 79.17 ab | 62.67 | 61.67 | 62.17 c | |
| N15 | 0.020 | 3 | 86.67 | 87.00 | 86.83 cdef | 77.67 | 74.33 | 76.00 bcd | 62.33 | 60.33 | 61.33 cde | |
| N16 | 0.020 | 4 | 83.33 | 88.00 | 85.67 defg | 76.00 | 72.67 | 74.33 cde | 58.67 | 56.00 | 57.33 fgh | |
| 85.42 b | 87.94 a | 75.96 a | 73.17 b | 61.81 a | 59.31 b | |||||||
|
LSD value: Genotype* = 1.4327 Treatments* = 4.0523 Genotype × Treatment NS = 5.7308 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05) |
LSD value: Genotype* = 1.4614 Treatments* = 4.1335 Genotype × Treatment NS = 5.8457 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05) |
LSD value: Genotype* = 1.3448 Treatments* = 3.8036 Genotype × Treatment NS = 5.3791 (*Significant;NS Non-significant) Values sharing common letters do not differ significantly (α = 0.05) |
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