FIGURE 5.

PYGB knockdown increases susceptibility to depression. (A) Timeline of AAV injection, chronic social defeat stress model establishment, behavioral tests, and immunohistochemistry. (B) Immunofluorescence staining was performed to identify the astrocyte-specific localization of exogenous PYGB in mice (n = 8). Scale bars = 20 μm. (C) Protein levels of PYGB and P-PYGB were analyzed via immunoblotting in the medial prefrontal cortex of Pygb-knockdown mice (n = 3). (D) Glycogen level and GP activity in astrocytic Pygb-knockdown mouse models (n = 5). (E) Pygb-knockdown mouse models were assessed by social interaction and tail suspension tests (n = 7). *p < 0.05, **p < 0.01. AAV, adeno associated virus; GP, glycogen phosphorylase; PYGB, glycogen phosphorylase; CTRL, control mouse models; KD-Pygb, Pygb-knockdown mouse models.