Figure 3:

A) Baseline systolic blood pressure in male and female mice (WT, M/F= 13/9; Myh11-4a12, M/F=15/12). Results are means ± SE; **p<0.01; ****p<0.0001 (2-way ANOVA). B) Baseline acetylcholine-induced relaxation in RIA (N=13 WT; N=15 Myh11-4a12). Results are means ± SE, ***p<0.001 (2-way ANOVA). C) Sodium nitroprusside (SNP)-induced relaxation in RIA (N=3 WT; N=4 Myh11-4a12). Results are means ± SE. D) Representative Western blot and densitometry analysis for Serine 1177 phospho-eNOS expression (N=6-7/group). Results are means ± SE; *p<0.05 (unpaired t-test). E) Media-to-lumen ratio, F) media thickness and G) media cross-sectional area (CSA) (N=12-13/group) Results are means ± SE **p<0.01, ***p<0.001, ****p<0.0001 (unpaired t-test)