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. 2022 Jan 24;9:801816. doi: 10.3389/fmolb.2022.801816

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Copyright © 2022 Jiménez-Alcántar, López-Gómez, López-Meza and Ochoa-Zarzosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PaDef antimicrobial peptide induces apoptosis in Jurkat acute lymphoid leukemia line. Representative flow cytometry plots of the different treatments are shown; (A) Vehicle (DMSO 0.98%); (B) PaDef (47.3 μM); (C) Actinomycin D 0.5 μM (Act-D). Annexin V/7AAD staining was used to establish the apoptotic rate. Quadrants indicate viable cells (lower left quadrant), early apoptosis (lower right quadrant), late apoptosis (upper right quadrant), and necrotic cells (upper left quadrant). (D) The graph shows the relative fluorescence units for each condition. Each bar shows the mean of triplicates ± SE of three independent experiments. A minimum of 7,000 events per sample was collected. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey´s comparison, p ≤ 0.05).