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. 2022 Jan 24;9:801816. doi: 10.3389/fmolb.2022.801816

FIGURE 4.

FIGURE 4

Antimicrobial PaDef peptide induces activation of caspases 8 and 9 in Jurkat cells. Cells were treated for 24 h with vehicle (DMSO 0.98%), PaDef (47.3 μM) and Actinomycin D (0.5 μM, Act-D). Cells were stained using FITC-IETD-FMK for caspase 8 (A) and FITC-LEHD-FMK for caspase 9 (B) and subsequently analyzed by flow cytometry. (C) Viability assay (MTT). Cells were treated for 24 h with vehicle (DMSO 0.98%), PaDef (47.3 μM), or Actinomycin D (0.5 μM) with or without Pan caspase inhibitor Z-VAD-FMK (added 1 h before the above treatments). Each bar shows the mean of triplicates ± SE of three independent experiments. * (p ≤ 0.05), ** (p ≤ 0.01), *** (p ≤ 0.001) and **** (p ≤ 0.0001) indicate the significant statistical difference compared between treatments (One-way ANOVA and Tukey’s pairwise comparison, p ≤ 0.05).