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. 2022 Jan 24;9:801816. doi: 10.3389/fmolb.2022.801816

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Copyright © 2022 Jiménez-Alcántar, López-Gómez, López-Meza and Ochoa-Zarzosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antimicrobial PaDef peptide induces loss of mitochondrial membrane potential (ΔΨm) in Jurkat cells. Representative plots are shown. Cells were treated for 24 h with (A) vehicle (DMSO 0.98%), (B) PaDef (47.3 μM) or (C) Actinomycin D (0.5 μM, Act-D). Cells were stained with JC-1 dye and fluorescence was measured by flow cytometry. (D) The graph shows the percentage of loss of membrane potential for each treatment. Each bar shows the mean of triplicates ± SE of three independent experiments. Different letters denote significant differences (the same letter denotes no difference) in all values compared with each other (One-way ANOVA and Tukey’s pairwise comparison, p ≤ 0.05).