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. 2022 Jan 24;9:801816. doi: 10.3389/fmolb.2022.801816

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Copyright © 2022 Jiménez-Alcántar, López-Gómez, López-Meza and Ochoa-Zarzosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PaDef induces reactive oxygen species (ROS) generation in the Jurkat cell line. Cells were treated for 24 h: PaDef (47.3 μM), 0.98% DMSO Vehicle or 12% ethanol. Cells were subsequently stained with the indicators DHE (dihydroethidium) (A) and DHR (dihydrorhodamine) (B) and analyzed by flow cytometry. A representative image of a flow cytometry plot is shown. Each bar shows the mean of triplicates ± SE of two independent experiments. Different letters indicate statistically significant differences based on the vehicle (p ≤ 0.05) and were compared using one-way ANOVA and Tukey post hoc test (p ≤ 0.05).