FIGURE 8.

Synergistic effect of PaDef and doxorubicin in Jurkat cells. (A) Effect of doxorubicin on the viability of Jurkat cells. Cells were treated under increasing concentrations of the drug (1, 2, 4 and 10 μM) for 24 h and analyzed by MTT. The data show the percentage of cell viability. Actynomycin D 0.5 μM (Act-D) was used as a positive death control. Data represent the mean of three independent experiments performed in triplicate. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey´s comparison, p ≤ 0.05). (B) The half-maximal inhibitory concentration (IC₅₀) was calculated by linear regression analysis; IC₅₀: 3 μM; R² = 0.9294. (C) Jurkat cells were subjected to the simultaneous treatment with PaDef (9.6, 29, 48, 67 and 87 μM) and doxorubicin (0.5, 1, 3, 6 and 9 μM) and incubated for 24 h. The viable cells were determined by MTT assay and the IC₅₀ was estimated. Data represent the mean of three independent experiments performed in triplicate. Different letters denote significant differences within the treatments (one-way ANOVA and Tukey´s comparison, p ≤ 0.05).