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. 2021 Sep 10;70(5):815–820. doi: 10.33549/physiolres.934666

Table 1.

Antibodies used for Western blot and immunofluorescence.

Antibodies used for Western blot

Primary Antibody Abbreviation Host Isotype Clonality Produced by
α-Smooth muscle actin α-SMA Rabbit IgG Monoclonal CST, Danvers, MA, USA
Fibronectin Fibr Rabbit IgG Monoclonal Abcam, Cambridge, UK
Phospho-ERK1/2 pERK1/2 Rabbit Polyclonal CST, Danvers, MA, USA
Phospho-AKT pAKT Rabbit IgG Monoclonal CST, Danvers, MA, USA
Phospho-SMAD3 pSMAD3 Rabbit IgG Monoclonal Abcam, Cambridge, UK
ERK1/2 ERK1/2 Rabbit IgG Monoclonal CST, Danvers, MA, USA
SMAD3 SMAD3 Rabbit IgG Monoclonal CST, Danvers, MA, USA
AKT AKT Rabbit Polyclonal CST, Danvers, MA, USA
β-Actin β-actin Rabbit IgG Monoclonal CST, Danvers, MA, USA
D-Glyceraldehyde 3-phosphate GAPDH Rabbit IgG Monoclonal CST, Danvers, MA, USA

Secondary Antibody Host Isotype Produced by

Anti-rabbit, HRP-linked goat IgG CST, Danvers, MA, USA

Antibodies used for immunofluorescence

Primary Antibody Abbreviation Host Produced by Secondary Antibody Produced by Channel

α-Smooth muscle actin α-SMA Mouse monoclonal DakoCytomation, Denmark Goat anti-mouse Sigma-Aldrich, St. Louis, MO, USA TRITC-red
Fibronectin Fibr Rabbit polyclonal DakoCytomation, Denmark Goat anti-rabbit Sigma-Aldrich, St. Louis, MO, USA FITC-green