Table 1.
Antibodies used for Western blot | ||||||
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Primary Antibody | Abbreviation | Host | Isotype | Clonality | Produced by | |
α-Smooth muscle actin | α-SMA | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA | |
Fibronectin | Fibr | Rabbit | IgG | Monoclonal | Abcam, Cambridge, UK | |
Phospho-ERK1/2 | pERK1/2 | Rabbit | Polyclonal | CST, Danvers, MA, USA | ||
Phospho-AKT | pAKT | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA | |
Phospho-SMAD3 | pSMAD3 | Rabbit | IgG | Monoclonal | Abcam, Cambridge, UK | |
ERK1/2 | ERK1/2 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA | |
SMAD3 | SMAD3 | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA | |
AKT | AKT | Rabbit | Polyclonal | CST, Danvers, MA, USA | ||
β-Actin | β-actin | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA | |
D-Glyceraldehyde 3-phosphate | GAPDH | Rabbit | IgG | Monoclonal | CST, Danvers, MA, USA | |
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Secondary Antibody | Host | Isotype | Produced by | |||
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Anti-rabbit, HRP-linked | goat | IgG | CST, Danvers, MA, USA | |||
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Antibodies used for immunofluorescence | ||||||
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Primary Antibody | Abbreviation | Host | Produced by | Secondary Antibody | Produced by | Channel |
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α-Smooth muscle actin | α-SMA | Mouse monoclonal | DakoCytomation, Denmark | Goat anti-mouse | Sigma-Aldrich, St. Louis, MO, USA | TRITC-red |
Fibronectin | Fibr | Rabbit polyclonal | DakoCytomation, Denmark | Goat anti-rabbit | Sigma-Aldrich, St. Louis, MO, USA | FITC-green |