Skip to main content
. Author manuscript; available in PMC: 2022 Aug 15.
Published in final edited form as: J Immunol. 2022 Jan 17;208(4):851–860. doi: 10.4049/jimmunol.2100653

Figure 2.

Figure 2.

CD80/CD86 signaling is critical for CD4+ MP and Treg cell homeostasis in both lymphoid and non-lymphoid sites, but the effects of mAb treatment are reversible. WT C57BL/6 mice were injected with either Rat-IgG2a or anti-CD28 dAb every other d for 6d, and lymphocytes from mesenteric lymph nodes (A-D) and liver (E-H) were harvested on d8. (A) Ki-67 expression on Ly-6C−, (B) Absolute number of Tregs on d8, (C) Ki-67 expression on CD4+Foxp3−CD44+ T cells, (D) Absolute number of CD4+Foxp3−CD44+ T cells. (E) Ki-67 expression on Ly-6C− Treg, (F) Absolute number of Tregs, (G) Ki-67 expression on CD4+Foxp3−CD44+ T cells, (H) Absolute number of CD4+Foxp3−CD44+ T cells. (I-P) C57BL/6 mice were injected Rat IgG2a or anti-CD80/CD86 on d0, d2, d4, and d6. Splenic cells were harvested on d30 or d45. (I & J) Percentage of CD4+Foxp3+ Treg and Ki-67 expression on Ly-6C− Treg on d30. (K & L) Percentage of Treg and Ki-67 expression on Ly-6C− Tregs on d45. (M & N) Absolute number of Treg and CD4+Foxp3−CD44+ T cells on d30 (O & P) Absolute number of Treg and CD4+Foxp3−CD44+ T cells on d45. *p, 0.05; **p; 0.005; ***p, 0.0005, ****p, 0.00005.