TABLE 1.
Tm of p66 and recA gene fragmentsa
| Species (strain) or patient no. | Diagnosis | Tm (°C) calculated | Mean Tm (± SD) (°C)
|
|
|---|---|---|---|---|
| p66 gene amplicon | recA gene amplicon | |||
| B. burgdorferi sensu stricto (B31) | 81.7 | 78.72 ± 0.28 | 84.67 ± 0.28 | |
| B. afzelii (NE 632) | 79.0 | 77.27 ± 0.33 | 84.20 ± 0.40 | |
| B. garinii | 79.0 | 77.24 ± 0.37 | 82.89 ± 0.49 | |
| 1 | EM | 77.65 | 84.24 | |
| 2 | EM | 77.37 | 84.26 | |
| 3 | EM | 77.57 | 84.62 | |
| 4 | EM | 77.63 | 84.26 | |
| 5 | ACA | 77.95 | NAb | |
| 6 | EM | 76.87 | NA | |
| 7 | EM | 77.15 | NA | |
| 8 | EM | 79.23 | NA | |
a
Tms of the p66 gene fragment were calculated by the oligoapplet program and obtained by LightCycler PCR analysis of the p66 gene amplicon and the recA gene amplicon of the three reference strains of B. burgdorferi sensu lato and of patients with EM or ACA. For the three reference strains, mean Tms ± SD for 10 independent experiments are given.
b
NA, no amplification of Borrelia DNA could be detected.