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. 2022 Jan 26;18(1):e1009784. doi: 10.1371/journal.ppat.1009784

Fig 1. Cholesterol efflux arrest and inhibition of ASFV infectivity.

Fig 1

(A) Treatment with increasing concentrations of U18666A chemical compound altered the distribution of unesterified cholesterol (CHO) in Vero cells (filipin; blue). Scale bar: 10 μM. (B) Cells pre-treated with increasing concentrations of U18666A compound and infected with recombinant ASFV BPP30GFP for 16 hpi. GFP fluorescence intensity was measured using a plate reader. Graph represents mean±sem of 3 independent experiments. Statistically significant differences are indicated by asterisks (****p<0.0001, *p<0.05). (C) Visualization of acidic vesicles with lysotracker (red) in Vero cells treated with U18666A drug or controls. These acidic vesicles were absent after treatment with lysosomotropic drugs (Scale bar: 25 μM).