. 2022 Feb 7;17(2):e0254465. doi: 10.1371/journal.pone.0254465

Table 2. Primers used to detect virulence gene in Salmonella isolates.

PCR	Target Gene	Sequence	Amplicon size (bp)	Thermal Profile	References
P-1	agfA	`F-TCCACAATGGGGCGGCGGCG`	350	94°C for 1 sec; 58°C for 1 sec; 74°C 21 sec	[59]
P-1	agfA	`R-CCTGACGCACCATTACGCTG`	350	94°C for 1 sec; 58°C for 1 sec; 74°C 21 sec	[59]
P-2	IpfA	`F-CTTTCGCTGCTGAATCTGGT`	250	94°C for 1 sec; 55°C for 1 sec; 74°C 21 sec	[37]
P-2	IpfA	`R-CAGTGTTAACAGAAACCAGT`	250	94°C for 1 sec; 55°C for 1 sec; 74°C 21 sec	[37]
P-3	hilA	`F-CTGCCGCAGTGTTAAGGATA`	497	94°C for 120 sec; 62°C for 1 min; 72°C 1 min	[60]
P-3	hilA	`R-CTGTCGCCTTAATCGCATGT`	497	94°C for 120 sec; 62°C for 1 min; 72°C 1 min	[60]
P-4	sivH	`F-GTATGCGAACAAGCGTAACAC`	763	94°C for 30 sec; 56°C for 45 sec; 72°C 45 sec	[61]
P-4	sivH	`R-CAGAATGCGAATCCTTCGCAC`	763	94°C for 30 sec; 56°C for 45 sec; 72°C 45 sec	[61]
P-5	sefA	`F-GATACTGCTGAACGTAGAAGG`	488	94°C for 1 sec; 56°C for 1 sec; 74°C 21 sec	[62]
P-5	sefA	`R-GCGTAAATCAGCATCTGCAGTAGC`	488	94°C for 1 sec; 56°C for 1 sec; 74°C 21 sec	[62]
P-6	sopE	`F-GGATGCCTTCTGATGTTGACTGG`	398	94°C for 1 min; 55°C for 1 min; 72°C for 1 min	[63]
P-6	sopE	`R-ACACACTTTCACCGAGGAAGCG`	398	94°C for 1 min; 55°C for 1 min; 72°C for 1 min	[63]
P-7	spvC	`F-CCCAAACCCATACTTACTCTG`	669	93°C for 1 min; 42°C for 1 min; 72°C for 2 min	[64]
P-7	spvC	`R-CGGAAATACCATCTACAAATA`	669	93°C for 1 min; 42°C for 1 min; 72°C for 2 min	[64]