Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Pdgfra | Santa Cruz | Cat#: sc-338; RRID: AB_631064 |
| Goat anti-Nestin | Santa Cruz | Cat#: sc-21248; RRID: AB_2148925 |
| Rabbit anti-Fa2h | Proteintech | Cat#: 15452-1-AP; RRID: AB_2101886 |
| Rabbit anti-Olig2 | Millipore | Cat#: Ab9610; RRID: AB_570666 |
| Rabbit anti-Gfap | Cell Signaling | Cat#: 12389T; RRID: AB_2631098 |
| Rabbit anti-KI67 | Novus | Cat# NB500-170; RRID:AB_1000197 |
| Mouse anti-TF(F3)(H-9) | Santa Cruz | Cat#: sc-374441; RRID: AB_11008609 |
| Mouse anti-CD142(F3) - FITC | Miltenyi Biotec | Cat# 130-098-854, RRID: AB_2655127 |
| Rabbit anti-Cleaved Caspase-3 (Asp175) | Cell Signaling | Cat# 9661, RRID: AB_2341188 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma | Cat#: T5648 |
| EGF | Fisher Scientific | Cat#: PHG0311 |
| bFGF | Gemini | Cat#: 300-113P 50ug |
| iScript cDNA synthesis kit | Biorad | Cat#: 1708890 |
| RNeasy Extraction Kit | Qiagen | Cat#: 74104 |
| Deposited Data | ||
| Neftel et.al., dataset | https://pubmed.ncbi.nlm.nih.gov/31327527/ | |
| Yuan et.al., dataset | https://pubmed.ncbi.nlm.nih.gov/30041684/ | |
| TCGA GBM RNAseq and microarray dataset | Cbioportal: https://www.cbioportal.org | GBM (TCGA 2003) |
| RNA sequencing data generated in this study | This paper | GEO: GSE190129 |
| Codes for 8 PDX data analysis | This paper | https://doi.org/10.7910/DVN/J5MVOR |
| Codes for Neftel et al., data analysis | This paper | https://doi.org/10.7910/DVN/FODWRV |
| Codes for 4 primary GBM data analysis from Yuan et al., | This paper | https://doi.org/10.7910/DVN/SYP8LH |
| Codes for 2 recurrent GBM data analysis from Yuan et al., | This paper | https://doi.org/10.7910/DVN/JXVB7R |
| Experimental Models: human GBM PDX Lines | ||
| PDX180327-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX180518-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX170911-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX160830-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX180709-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX180911-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX160810-1; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| PDX161129-2; GBM patient-derived primary tumor xenografts | Established at MSKCC (Brain Tumor Center) | N/A |
| Experimental Models: Organisms/Strains | ||
| CGD | https://pubmed.ncbi.nlm.nih.gov/33229571/ | N/A |
| Nf1 flox | https://pubmed.ncbi.nlm.nih.gov/11297510/ | N/A |
| Trp53 flox | https://pubmed.ncbi.nlm.nih.gov/15150107/ | N/A |
| Pten flox | https://pubmed.ncbi.nlm.nih.gov/11691952/ | N/A |
| Oligonucleotides | ||
| GAPDH-Forward: TTTGGCTACAGCAACAGGGT | Eton Bioscience | N/A |
| GAPDH-Reverse: CCAGCAGTGAGGGTCTCTCT | Eton Bioscience | N/A |
| F3-Forward: GGAAGAGTACAGACAGCCCG | Eton Bioscience | N/A |
| F3- Reverse: ACCACAAATACCACAGCTCCA | Eton Bioscience | N/A |
| TIMP3-Forward: GGCACGCTGGTCTACACC | Eton Bioscience | N/A |
| TIMP3-Reverse: TGGTACTTGTTGACCTCCAGC | Eton Bioscience | N/A |
| ID3-Forward: GGCTAAGCTGAGTGCCTCTC | Eton Bioscience | N/A |
| ID3-Reverse: AGCTTGCTGGACGACATGAA | Eton Bioscience | N/A |
| APOE-Forward: TTTGGGATTACCTGCGCTGG | Eton Bioscience | N/A |
| APOE-Reverse: TCATGGTCTCGTCCATCAGC | Eton Bioscience | N/A |
| EDNRB-Forward: TCAGTGAAGCCATGTTGATACCA | Eton Bioscience | N/A |
| EDNRB-Reverse: TGGTCCTTGTCTTTGCCCTC | Eton Bioscience | N/A |
| KI67-Forward: ACCAAGCTTTGTGCCTTCAC | Eton Bioscience | N/A |
| KI67-Reverse: AATGCACACTCCACCTGTCC | Eton Bioscience | N/A |
| Software and Algorithms | ||
| GraphPad Prism v.7 | www.graphpad.com | RRID: SCR_002798 |
| Image J | www.imagej.net | RRID: SCR_003070 |
| cBioPortal | www.cBioPortal.org | RRID: SCR_014555 |
| GSEA | http://software.broadinstitute.org/gsea/index.jsp | RRID: SCR_003199 |
| Bioconductor | http://www.bioconductor.org | RRID: SCR_006442 |
| Limma | https://www.bioconductor.org/packages/release/bioc/html/limma.html | RRID: SCR_010943 |
| DESeq2 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html | RRID: SCR_015687 |
| Seurat | https://satijalab.org/seurat/articles/get_started.html | RRID: SCR_016341 |
| AUCell | https://bioconductor.org/packages/release/bioc/html/AUCell.html | RRID: SCR_021327 |