Table 4.

Oxygen-glucose deprivation/reperfusion (OGD/R) induces death of PC12 cells

Treatments	Injury duration	Reperfusion duration	Death type	Assessment	Critical protein	Reference
OGD/R	12 h	1 h	Pyroptosis	Hoechst 33342, PI, CCK-8	NLRP3, C-CASP-1, GSDMD-N	Zeng et al., 2020
OGD/R	2 h	2 h	Pyroptosis	MTT, LDH, TUNEL	CASP-1, CASP-1p20, GSDMD, GSDMD-N	Li et al., 2021b
OGD/R	12 h	4 h	Pyroptosis	MTT, TEM	NLRP3, ASC, C-CASP-1, GSDMD, GSDMD-N	Liu et al., 2021a
OGD	2, 4, 8, 12, 24 h	0	Apoptosis	MTT	C-CASP-3, Bcl-2, Bax	Lin et al., 2015
OGD	12 h	0	Apoptosis	Hoechst 33342, MTT, Flow cytometry	C-CASP-3, CASP-12, Bcl-2	Cao et al., 2016
OGD/R	0, 2, 4 h	24 h	Apoptosis	TUNEL, CCK-8	Bcl-2, Bax	Ren et al., 2019
OGD	8 h	0	Necroptosis	PI (Nec-1)	RIP1, RIP3, MLKL	Wang et al., 2018
H/R	8 h	24 h	Necroptosis	LDH, Flow cytometry	RIP1, RIP3, MLKL/p-MLKL	Zhang et al., 2019b

ASC: Apoptosis-associated speck-like protein containing a caspase recruit domain; Bax: B-cell lymphoma 2-associated X; Bcl-2: B-cell lymphoma 2; CASP: cysteinyl aspartate-specific protease; C-CASP: cleaved CASP; CCK-8: cell counting kit-8; C-GSDMD: cleaved GSDMD; GSDMD: gasdermin D; GSDMD-N: N-terminal domain of gasdermin D; H/R: Hypoxia/reoxygenation; IL: interleukin; LDH: lactate dehydrogenase; MLKL: mixed lineage kinase domain-like pseudokinase; MTT: methylthiazolyldiphenyl-tetrazolium bromide; Nec-1: Necrostatin-1; NLRP: nucleotide-binding domain and leucine-rich repeat pyrin-domain containing protein; OGD: oxygen and glucose deprivation; OGD/R: OGD/recovery; PI: propidium iodide staining; PI (Nec-1): Nec-1 inhibited necroptosis, and after the addition of Nec-1, PI staining was performed to verify the occurrence of necroptosis by comparing with the control group; p-MLKL: phosphorylation of MLKL; RIP: receptorinteracting protein kinase; SD rats: Sprague-Dawley rats; TEM: transmission electron microscope; TUNEL: TdT-mediated dUTP nick-end labeling.