Figure 2.

Crry protein expression increases with age in an Alzheimer’s disease context.

(A, C) Crry, Iba-1, and β-actin protein expression in the cortex and hippocampus of P301S mice and age-matched WT mice at different ages, as visualized using western blot. (B, D–F) Quantification of Crry and Iba-1 protein levels in the cortex and hippocampus of P301S mice and age-matched WT mice. (G, H) Crry expression was significantly upregulated in the cortex and hippocampus of 6-month-old P301S mice after normalizing Crry expression to microglial Crry expression. Protein expression levels were normalized to β-actin. Data are represented as the mean ± SEM (n = 6 for each group) and were analyzed by one-way analysis of variance followed by Tukey’s multiple comparison test (B, D–F) and by independent sample t-test (G, H). †P < 0.05. Crry: Cr1-related protein Y; Iba-1: ionized calcium binding adaptor molecule 1; WT: wild type.