Figure 4.

Hyperphosphorylated tau is decreased after infusion with Crry shRNA in the brains of 6-month-old P301S mice.

(A) Levels of AT8 (which recognizes serine 202 and threonine 205 hyperphosphorylated tau), threonine (thr) 231 hyperphosphorylated tau, serine (ser) 262 hyperphosphorylated tau, and total tau were measured by western blot. (B–D) Quantification of the proportion of AT8/total tau (B), threonine 231 hyperphosphorylated tau/total tau (C), and serine 262 hyperphosphorylated tau/total tau (D). Protein expression levels were normalized to β-actin. Data are presented as the mean ± SEM (n = 6 for each group) and were analyzed by one-way analysis of variance followed by Tukey’s multiple comparison test. †P < 0.05. Crry: Cr1-related protein Y; shRNA: short hairpin RNA; WT: wild type.