Addtional Table 1.
Antibodies used in western blot and double immunofluorescence staining
| Antibody | Concentration | RRID No. | Species and clonality | Catalog No. | Supplier |
|---|---|---|---|---|---|
| Crry | 1:200 | AB_2085152 | Rabbit monoclonal | sc-30214 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Iba-1 | 1:500 | AB_2636859 | Rabbit monoclonal | ab178846 | Abcam, Cambridge,UK |
| GFAP | 1:500 | AB_305808 | Rabbit polyclonal | ab7260 | Abcam, Cambridge,UK |
| NeuN | 1:1000 | AB_2532109 | Rabbit monoclonal | ab177487 | Abcam, Cambridge,UK |
| OSP | 1:500 | AB_305935 | Rabbit polyclonal | ab7474 | Abcam, Cambridge,UK |
| Hyperphosphorylated tau at Ser202:Thr205 (AT8) | 1:1000 | AB_223647 | Mouse monoclonal | mn1020 | ThermoFisher Scientific, Sunnyvale, CA, USA |
| Hyperphosphorylated tau at Thr231 | 1:1000 | AB_2533742 | Rabbit polyclonal | 44-746G | ThermoFisher Scientific, Sunnyvale, CA, USA |
| Hyperphosphorylated tau at Ser262 | 1:1000 | AB_2533743 | Rabbit polyclonal | 44-750G | ThermoFisher Scientific, Sunnyvale, CA, USA |
| Total tau | 1:1000 | AB_628327 | Mouse monoclonal | sc-32274 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Phospho-GSK-3β (Ser9) | 1:1000 | AB_2798546 | Rabbit monoclonal | 5558T | Cell Signaling Technology, Danvers, MA, USA |
| GSK-3α:β | 1:1000 | AB_2636978 | Rabbit monoclonal | 12456T | Cell Signaling Technology, Danvers, MA, USA |
| AMPK | 1:1000 | AB_1118940 | Mouse monoclonal | sc-74461 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Phospho-AMPKα (Thr172) |
1:1000 | AB_2799368 | Rabbit monoclonal | 50081s | Cell Signaling Technology, Danvers, Massachusetts, USA |
| Phospho-p38 MAPK (Thr180:Tyr182) |
1:1000 | AB_2139682 | Rabbit monoclonal | 4511T | Cell Signaling Technology, Danvers, MA, USA |
| p38 MAPK | 1:1000 | AB_2533144 | Mouse monoclonal | 33-8700 | ThermoFisher Scientific, Sunnyvale, CA, USA |
| JNK | 1:1000 | AB_2140722 | Mouse monoclonal | sc-137019 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Phospho-JNK (Thr183:Tyr185) |
1:1000 | AB_2574777 | Mouse monoclonal | sc-293136 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| CDK5 | 1:1000 | AB_627241 | Mouse monoclonal | sc-6247 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Synaptophysin | 1:1000 | AB_2286949 | Rabbit monoclonal | ab32127 | Abcam, Cambridge,UK |
| PP2Ac | 1:1000 | AB_2170107 | Mouse monoclonal | sc-81603 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| C3 | 1:500 | AB_2066623 | Rat monoclonal | ab11862 | Abcam, Cambridge,UK |
| C3b | 1:2000 | AB_627277 | Mouse monoclonal | sc-28294 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Phospho-CaMKII-α (Tyr231) |
1:1000 | AB_2070310 | Rabbit polyclonal | 3356s | Cell Signaling Technology, Danvers, MA, USA |
| CaMKII-α | 1:1000 | AB_2721906 | Mouse monoclonal | 50049s | Cell Signaling Technology, Danvers, MA, USA |
| Cleaved caspase-3 | 1:500 | AB_2341188 | Rabbit polyclonal | 9661 | Cell Signaling Technology, Danvers, MA, USA |
| β-Actin | 1:1000 | AB_2714189 | Mouse monoclonal | sc-47778 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
| Goat anti-Mouse IgG (H+L) Poly-HRP Secondary Antibody |
1:1000 | AB_1965958 | Polyclonal | 32230 | ThermoFisher Scientific, Sunnyvale, CA, USA |
| Fluorescein-conjugated affinipure Goat anti-Rabbit IgG antibody | 1:200 | AB_2571576 | Polyclonal | ZF-0311 | Zhongshan Goldenbridge Inc., Beijing, China |
| FITC labeled Goat anti-mouse IgG antibody | 1:200 | AB_2716306 | Polyclonal | ZF-0312 | Zhongshan Goldenbridge Inc., Beijing, China |
AMPK: AMP-activated protein kinase; C3: complement 3; C3b: complement component 3b; CaMKII-α: CaM-dependent protein kinase II-α; CDK5: cyclin-dependent kinase 5; Crry: Cr1-related protein Y; GFAP: glial fibrillary acidic protein; GSK: glycogen synthase kinase; Iba-1: ionized calcium binding adaptor molecule 1; JNK: c-Jun N-terminal kinase; MAPK: mitogen-activated protein kinases; NeuN: neuronal nuclei; OSP: oligodendrocyte specific protein; PP2Ac: protein phosphatase 2A catalytic subunit.