Figure 4.

Evaluation of the reactive oxygen species (ROS) generation and biocompatibility of Ta₄C₃T _x MQDs. A) Total cellular ROS was evaluated in HUVECs using a green fluorescence probe. B) Culture with Ta₄C₃T _x MQDs at concentrations of 2 to 100 µg mL⁻¹ did not increase total cellular ROS when compared to the control. C) Additionally, caspase‐3/7 activation was assessed using a green fluorescent probe, and Ta₄C₃T _x MQDs at concentrations of 2 to 100 µg mL⁻¹ did not increase apoptotic activation when compared to the control. D) Biocompatibility at 3 days was assessed at different MQD concentrations (0.5 to 20 µg mL⁻¹) using the LDH release assay. No significant increases were observed in maximum LDH release between MQD‐treated groups and the control. E) Biocompatibility at 7 days was assessed at different MQD concentrations (0.5 to 20 µg mL⁻¹) using the WST‐1 Cell Proliferation assay. No significant differences were observed in cellular proliferation between the MQD‐treated groups and the control.