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. 2022 Jan 21;11:e67576. doi: 10.7554/eLife.67576

Figure 7. Bihormonal cells can also arise in the pancreatic ducts.

(A–B) Whole mount immunodetection of GFP that highlights the ducts (green), mCherry (red) for β-cells and Sst (gray) on the entire pancreas of Tg(nkx6.1:eGFP); Tg(ins:NTR-P2A-mCherry) larvae at 17 dpf. (A) CTL larvae showing the main islet in the head and a few monohormonal endocrine cells (mCherry+ or Sst+) in the ductal GFP+ domain in the tail. The pancreatic tail is delineated by white dashed lines. (B) After treatment with NFP from 3 to 4 dpf, regenerating larvae display scattered bihormonal cells (red and gray) in the tail along the ducts. Stacks represent 3D projections of confocal images of the whole pancreas. (B’) Close-ups of two individual bihormonal cells in the tail (z-planes showing one unique optical section). (C) Quantification of Sst + mCherry + bihormonal cells based on confocal images. Mann-Whitney test, ****p < 0.0001. (See also Figure 7—source data 1).

Figure 7—source data 1. Bihormonal cell quantification in the tail (larvae).

Figure 7.

Figure 7—figure supplement 1. Time course of normal β and sst1.1 δ-cells differentiation from intrapancreatic ducts in the tail of Tgnkx6.1:eGFP; Tg(ins:NTR-P2A-mCherry) control larvae.

Figure 7—figure supplement 1.

Illustrative whole mount immunodetection on the whole pancreas of non-ablated (CTL) Tgnkx6.1:eGFP; Tg(ins:NTR-P2A-mCherry) larvae labeled with GFP to identify the pancreatic ductal domain (dotted lines), mCherry (red) and Sst (gray). Monohormonal mCherry+ and Sst + cells in the ducts were quantified from 7 to 17 dpf based on the confocal images.