Table 3. Affinity, occupancy number, and thermodynamic data calculated from isothermal titration calorimetry.
Biosensor | KD (μM) | n | ΔH(kcal/mol) | -TΔS(kcal/mol) | ΔG(kcal/mol) |
---|---|---|---|---|---|
iCytSnFR | 13.7 ± 1.1 | 0.84 ± 0.05 | –2.1 ± 0.1 | –4.6 ± 0.2 | –6.6 ± 0.1 |
iCyt_F_SnFR | 1.8 ± 0.5 | 0.83 ± 0.02 | –5.5 ± 0.1 | –2.4 ± 0.2 | –7.9 ± 0.1 |
iCyt_BrEt_SnFR | 5.4 ± 0.8 | 0.69 ± 0.09 | –1.12 ± 0.03 | 6.1 ± 0.1 | –7.2 ± 0.1 |
iDianiSnFR | 7.6 ± 1.4 | 0.92 ± 0.02 | 3.2 ± 0.5 | 10.1 ± 0.4 | –7.0 ± 0.2 |