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. 2022 Jan 4;11:e74648. doi: 10.7554/eLife.74648

Table 3. Affinity, occupancy number, and thermodynamic data calculated from isothermal titration calorimetry.

Data are the mean ± SEM, three runs.

Biosensor KD (μM) n ΔH(kcal/mol) -TΔS(kcal/mol) ΔG(kcal/mol)
iCytSnFR 13.7 ± 1.1 0.84 ± 0.05 –2.1 ± 0.1 –4.6 ± 0.2 –6.6 ± 0.1
iCyt_F_SnFR 1.8 ± 0.5 0.83 ± 0.02 –5.5 ± 0.1 –2.4 ± 0.2 –7.9 ± 0.1
iCyt_BrEt_SnFR 5.4 ± 0.8 0.69 ± 0.09 –1.12 ± 0.03 6.1 ± 0.1 –7.2 ± 0.1
iDianiSnFR 7.6 ± 1.4 0.92 ± 0.02 3.2 ± 0.5 10.1 ± 0.4 –7.0 ± 0.2