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. 2022 Feb 6;19(1):234–246. doi: 10.1080/15476286.2021.2021676

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Phosphorylation of CNOT2 after osmotic stress.

(A) HeLa cells were treated with sorbitol. Lysates were prepared at the indicated times after treatment, and analysed by immunoblot. (B) HeLa cell lysates in (A) were separated on Phos-tag SDS-PAGE and analysed by immunoblot using anti-CNOT2 antibody. (C) HeLa cells were pretreated with inhibitors for 15 min and then treated with sorbitol for an additional 30 min. Cell lysates were separated on SDS-PAGE or Phos-tag SDS-PAGE, followed by immunoblot. SP: SP600125 (JNK inhibitor), SB: SB203580 (p38MAPK inhibitor).