Figure 5. Structures of clickable probes for protein S-palmitoylation.
The native structures of palmitic acid and palmitoyl-CoA along with their azide- and alkyne-modified analogues are shown. Varying chain lengths can serve as surrogate for this lipid modification and hence can be generalized as S-acylation. The presence of a diazirine moiety in Alk-Dzn-C18 allows for a photocrosslinking strategy that enables identification of proteins that interact with fatty acylated proteins through the lipid modification.