Fig. 3.

γH2AX (Ser139), p-ATM (Ser1981), and p-CHK2 (Thr68) can be used as biomarkers to identify Ataxia Telangiectasia (AT). PBMCs of seven patients affected by AT and 21 healthy controls were irradiated with 2 Gy and fixed after 1 h, 4 h, 8 h, and 24 h. Mean fluorescence intensities (MFI) of γH2AX (Ser139), p-ATM (Ser1981), and p-CHK2 (Thr68) were analyzed by flow cytometry (FACSAria™, BD) and fold inductions were calculated based on MFIs obtained from unirradiated samples. Fold inductions are shown for γH2AX (A), p-ATM (B), and p-CHK2 (C) in controls and patients. Means are represented by horizontal bars, statistics were calculated using two-way ANOVA and Sidaks multiple comparison test; **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05