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. 2022 Jan 25;12:816231. doi: 10.3389/fimmu.2021.816231

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Copyright © 2022 Lia, Di Vito, Bruno, Tapparo, Brunello, Santoro, Mariotti, Bramanti, Zaghi, Calvi, Comba, Fascì, Giaccone, Camussi, Boyle, Castagna, Evangelista, Mavilio and Bruno

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of acute GvHD onset on the kinetics of EV miRNA. Proportional change of miR100, miR155, and miR194 (from top to bottom) quantified by real-rime PCR from precipitated EVs at different time points before and after aGvHD onset and compared to the pre-transplant baseline values. Dash black line: pre-transplant levels; dashed red line: time of aGvHD onset; circle and star dots represent outliers (>1.5 box length from median) and extreme values (>3 box length from median), respectively. Significant mean differences before the onset (p ≤ 0.05) between patients with aGvHD (red) and without (blue) are indicated.