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. 2022 Jan 25;9:813249. doi: 10.3389/fnut.2022.813249

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Copyright © 2022 Xu, Li, Yao, Xie, Chi, Zhang, Cao and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The activities of enzymes and expression of proteins in the liver samples. (A–E) the activities of LPS (lipase), CPT1 (carnitine palmitoyltransferase 1), ATGL (triglyceride lipase), ACC (acetyl-CoA carboxylase), and FAS (fatty acid synthase) in fish (n = 6). (F) the Western Blot analysis of SREBP1 (sterol responsive element binding protein 1), PPARA (peroxisome proliferator-activated receptor alpha), P-PPARA, and GAPDH in the liver (n = 3). (G–K) the relative quantification of FXR, TGR5, SREBP1, PPARA, and P-PPARA proteins normalized to the GAPDH level (n = 3). Values are presented as means with SD, where significant (p < 0.05) differences between groups are indicated by different letters. “*” indicates significant (p < 0.05) differences in the HD group (compared to the CD group), and “#” indicates significant (p < 0.05) differences in the B3D group (compared to the HD group).