Table 1.
Composition and concentration of nutrients in dietsa.
| Ingredients (%) | Test diets | ||||||
|---|---|---|---|---|---|---|---|
| CD | HD | B1D | B2D | B3D | B4D | B5D | |
| Fish meal | 50.00 | 50.00 | 50.00 | 50.00 | 50.00 | 50.00 | 50.00 |
| Vital wheat gluten | 11.50 | 11.50 | 11.50 | 11.50 | 11.50 | 11.50 | 11.50 |
| Wheat flour | 15.00 | 15.00 | 14.97 | 14.94 | 14.91 | 14.88 | 14.85 |
| Cottonseed protein | 7.32 | 7.32 | 7.32 | 7.32 | 7.32 | 7.32 | 7.32 |
| Corn gluten meal | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Fish oil | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Soybean oil | 1.50 | 7.50 | 7.50 | 7.50 | 7.50 | 7.50 | 7.50 |
| Soybean lecithin | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Calcium monophosphate | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Vitamin C | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 |
| Choline chloride | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Vitamin premixb | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Mineral premixc | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Antioxidant | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 | 0.05 |
| Attractant | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 | 0.10 |
| Cellulose microcrystalline | 6.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Taurocholic acid sodiumd | 0.00 | 0.00 | 0.03 | 0.06 | 0.09 | 0.12 | 0.15 |
| Proximate composition (% air dry matter) | |||||||
| Crude protein | 47.48 | 47.53 | 47.62 | 47.51 | 47.42 | 47.45 | 47.64 |
| Crude lipid | 8.27 | 14.94 | 15.32 | 15.66 | 15.56 | 15.60 | 15.46 |
| Crude ash | 11.50 | 11.21 | 11.50 | 11.29 | 11.37 | 11.29 | 11.36 |
| Moisture | 8.94 | 10.01 | 9.55 | 9.71 | 7.97 | 11.16 | 9.78 |
Seven diets: CD (control), HD (high-lipid), B1D (taurocholic acid sodium additional level at 300 mg kg−1), B2D (600), B3D (900), B4D (1,200), and B5D (1,500).
Vitamin mixture (g/kg mixture): vitamin B1, 17.00 g; vitamin B2, 16.67 g; vitamin B6, 33.33 g; vitamin B12, 0.07 g; vitamin K, 3.33 g; vitamin E, 66.00 g; retinyl acetate, 6.67 g; vitamin D, 33.33 g, nicotinic acid, 67.33 g; D-calcium pantothenate, 40.67 g; biotin, 16.67 g; folic acid, 4.17 g; inositol, 102.04 g; cellulose, 592.72 g. All ingredients were diluted with corn starch to 1 kg.
Mineral mixture (g/kg mixture): CaCO3, 350 g; NaH2PO4·H2O, 200 g; KH2PO4, 200 g; NaCl, 12 g; MgSO4·7H2O, 10g; FeSO4·7H2O, 2 g; MnSO4·7H2O, 2 g; AlCl3·6H2O, 1 g; CuCl2·2H2O, 1 g; KF, 1 g; NaMoO4·2H2O, 0.5 g; NaSeO3, 0.4 g; CoCl2·6H2O, 0.1 g; KI, 0.1 g; zeolite powder, 219.9 g. (Obtained from Zhanjiang Yuehai Feed Co. Ltd., Guangdong, China).
The measure value of taurocholic acid sodium: CD (no detected), HD (130.00 mg kg−1), B1D (393.30), B2D (659.90), B3D (888.10), B4D (1,197.90), and B5D (1,502.10).