Figure 2.

Correlation between RNF135 methylation and mRNA expression in HCC cell lines. (A) The methylation status in immortalized normal liver cell line LO2 and HCC cell lines (Bel-7402, HepG2, Huh7, MHCC-97H, MHCC-97L, SMMC-7721, SNU-387, and SNU-449) were measured by MS-PCR (M, methylation; U, unmethylation). H₂O was used as a negative control. (B) The expression levels in cell lines were determined by semi-quantitative RT-PCR, and H₂O was used as a negative control. (C) RNF135 mRNA expression was partially restored after treatment with the demethylation reagent decitabine in 4 HCC cell lines whose promoter region was hypermethylated. (D–F) Wound healing assay and transwell assay were used to detect cell migration and invasion (up), and one-way ANOVA was applied between experimental group and control group (down). ****P < 0.0001, ns represents no significance.