Figure 4.

RNF135 expression associations with immune infiltration in HCC tissues. (A) The enrichment of functions and signaling pathways of the target genes was conducted by Metascape. (B) The correlation between RNF135 expression and TMB (Spearman’s correlation r = −0.2411, p-value <0.001). (C) ESTIMATE was used to predict the association between RNF135 expression with the presence of infiltrating stromal/immune cells in tumor tissues: stromal score (quantifies the presence of stroma in tumor tissue); immune score (that represents the infiltration of immune cells in tumor tissue). (D) The associations between RNF135 expression with tumor purity and six immune cell types (B cell, CD8⁺ T cell, CD4⁺ T cell, macrophage, neutrophil, and dendritic cell) in the tumor microenvironment were estimated by TIMER.