Figure 6.

The JNK signaling pathway was activated in colon cancer cells after treated with IATL. (A, B) Phospho-JNK (p-JNK) and the total protein expression (JNK) in HCT116 (A), and HCT-15 (B) cells upon IATL treatment was examined by western blot analysis. (C–E) HCT116 and HCT-15 cells were treated with various dosages of IATL for 6 h, then the expression of p-JNK and JNK were examined by western blot analysis. (F–H) Cells were pretreated with NAC (5 mM) for 1 h and then treated with IATL (40 µM) for 6 h, the expression of p-JNK and JNK were examined by western blot analysis. (I, J) Cells were pretreated with SP600125 (20 µM) for 1 h and then treated with IATL (40 µM) for 6 h, the expression of p-JNK and JNK were examined by western blot analysis. (K) Cells were pretreated with SP600125 (20 µM) for 1 h and cell viability was measured after treated with IATL (40 µM) for 24 h. Data from three technical replicates (*p < 0.05, **p < 0.01, ***p < 0.001 vs DMSO; ^#p < 0.05 vs IATL-40).