Fig. 2. Diapause arrest at multiple stages.

a, b Schematic representation of the experimental design. Transient, GFP + clones are generated by heat shock (see methods for details). a As egg chambers develop, those containing GFP + cells mature into eggs that are laid, and are replaced by unlabeled egg chambers. b In diapause, if egg chambers are continually produced and degenerating (option 1, top), GFP labeling would be lost, but if egg chamber development stops (option 2, bottom), degenerating GFP-labeled egg chambers would persist. In either case, upon transfer to recovery conditions, development should resume and GFP labeling should disappear. c–e Controls showing the presence of transient GFP clones 1 day after heat shock (c) and loss thereafter (d, e). f–h In diapause, GFP-labeled egg chambers persist for six weeks and are lost after a week of recovery. Scale bars 100 µm. i Quantification of the percentage of ovarioles containing any egg chamber younger than stage 14 with GFP + cells, comparing normal aging to diapause and recovery. The data shown are from three independent experiments. Error bars = mean ± s.e.m. n is the total number of ovarioles analyzed. See also Supplementary Fig. 2.