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. 2022 Feb 7;12:2005. doi: 10.1038/s41598-022-06052-7

Figure 2.

Figure 2

The knockdown of caspase-1, caspase-4 and NLRP3 in bladder epithelial cells using the CRISPR/Cas9 system was assessed by Western blot analysis. GAPDH was used as loading control (A). Original western blot presented is available in Supplemental Fig. S1. IL-1β (B), IL-18 (C), IL-8 (D) and LDH (E) release from Cas9, caspase-1, caspase-4 or NLRP3-knockdown bladder epithelial cells after stimulation with UPEC strain CFT073 at MOI 10 for 6 h. gRNA stands for guideRNA targeting specific gene using CRISPR/Cas9. Data are presented as mean ± SEM of three independent experiments. Asterisks over error-bar show statistical significance compared to respective unstimulated control (*p < 0.05, **p < 0.01, ***p < 0.001).