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. 2022 Feb 7;12:2005. doi: 10.1038/s41598-022-06052-7

Figure 3.

Figure 3

Gene expression of inflammasome-related genes. Caspase-1, caspase-4 and NLRP3-knockdown bladder epithelial cells and Cas9 controls were infected with CFT073 followed by analysis of mRNA expression of IL-1β (A), IL-1α (B), IL-18 (C), IL-6 (D) and IL-8 (E). The gene expression was normalized to GAPDH and expressed as fold change relative to unstimulated controls. gRNA stands for guideRNA targeting specific gene using CRISPR/Cas9. Data are presented as mean ± SEM of three independent experiments. Asterisks denote statistical significance compared to Cas9 control cells (*p < 0.05, **p < 0.01, ***p < 0.001).