Figure 6.

Bacterial phagocytosis, production of reactive oxygen species (ROS) and bladder epithelial cell proliferation. Neutrophils were exposed to conditioned medium from Cas9 controls, caspase-1, caspase-4 and NLRP3-knockdown bladder epithelial cells and phagocytosis of CFT073 (eGFP) (3 h, A, B) and ROS-production was evaluated (C). Conditioned medium from respective knockdown cell lines was added to wild-type epithelial cells and proliferation was then evaluated after 24 h (D). Phagocytosis is expressed as fold change (mean florescence intensity) relative to the unstimulated control. A representative flow cytometry histogram illustrating neutrophils with (grey) or without (black) phagocytosis of CFT073 is shown in B. ROS production is presented as % of unstimulated control. gRNA stands for guideRNA targeting specific gene using CRISPR/Cas9. Data are presented as mean ± SEM of three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001).