Figure 5.
When overexpressed in hMSCs, TBX3 promotes bypass of senescence. (A) Senescence-Associated (SA)-β-Galactosidase staining of EV and TBX3 hMSCs. Representative brightfield microscopy images (200X; EVOS M5000 Imaging System; scale bars = 50 µm) are shown. (B) qRT-PCR and (C) Western blot analysis show p16INK4a mRNA and protein expression respectively in EV and TBX3 hMSCs. (C) β-actin was used as a loading control. Densitometry readings were obtained using Fiji and protein expression levels are represented as a ratio of protein of interest/β-actin normalized to EV #1. Student’s t-test was used to compare between groups, **p < 0.01; ***p < 0.001; error bars represent mean ± SEM (n=3, for all panels).