Figure 2.

VBIT-12 protects against DSS-induced UC pathology

(A) Experimental protocol for DSS-induced colitis (see Materials and methods). Mice (8 mice/group) were provided with 2% DSS in drinking water for 5 days. VBIT-12 (20 mg/kg) was given from day 2 (D2) or day 5 (D5), after the start of DSS treatment. On day 14, mice were sacrificed and monitored for colitis. (B) Representative images of colons, (C) colon length and (D) spleen weight. (E) The disease activated index (DAI) and the inflammation score (see Materials and methods section). The p value in blue color represents the significance relative to DSS-treated mice. (F) Representative images of colon tissue sections stained with H&E with superficial inflammation labeled by a dashed line. Scale bar is 50 μm. (G) Ki-67 staining; Scale bar, 50 μm. (H) Quantitative analysis of Ki-67. Red, yellow, blue, white, and orange arrows point to crypts, lamina propria, submucosa, lumen, and the muscularis mucosae, respectively. Results show means ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, with p value in blue color represents the significance relative to DSS-treated mice. NS, not significant.