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. 2022 Jan 25;12:812572. doi: 10.3389/fphys.2021.812572

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Copyright © 2022 Li, Loen, van Ham, Kool, van der Heyden and Takanari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nocodazole, Cytochalasin B or Dyngo-4a treatment up to 6 h do not affect K_IR2.1-Dendra2 protein expression level. (A) Western blot analysis of K_IR2.1-Dendra2 expression in HeLa Cells treated for different time period with Dyngo-4a (10 nM), Nocodazole (30 μM) or Cytochalasin B (20 μM) (n = 5). Non-transfected cells (NT) were used as negative control. (B) Western blot analysis of K_IR2.1-Dendra2 expression in CHO cells (n = 6). (C) Summarized results of K_IR2.1-Dendra2 expression in control and HeLa cells treated for 3 h or 6 h (n = 5). Data are presented as the fold of control. (D) Summarized results of (B). Data are showed as mean ± SD, control protein level was designed as 100% after correction. Ponceau staining was used as a loading control.