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. 2022 Jan 25;12:812572. doi: 10.3389/fphys.2021.812572

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Copyright © 2022 Li, Loen, van Ham, Kool, van der Heyden and Takanari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of changes in green and red fluorescence of K_IR2.1-Dendra2. (A,B) Summarized data of time-dependent recovery in green fluorescence (A) and decay of red fluorescence (B). Black boxes are for control (n = 14), and red circles, blue triangles, green triangles are for the data from the cells treated with Dyngo-4a (n = 15), Nocodazole (n = 20), Cytochalasin B (n = 17), respectively. (C,D) Summarized data of τ1 (C) and τ2 (D), fast and slow component of time constant for the recovery of green fluorescence after photoconversion, respectively. (E,F) Summarized data of τ1 (E) and τ2 (F), fast and slow component of time constant for the decay of red fluorescence after photoconversion, respectively. (A, inset) The maximum recovery rate of green fluorescence after photoconversion. *: p < 0.05 vs. control (one-way ANOVA).