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. 2022 Jan 25;12:812572. doi: 10.3389/fphys.2021.812572

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Copyright © 2022 Li, Loen, van Ham, Kool, van der Heyden and Takanari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Presence of K_IR2.1-GFP in the cytoskeleton containing cell fraction, induced by Chloroquine treatment. (A) Western blot analysis of whole cell lysates (WC), cytosol and membrane fractions (CM) and cytoskeleton fractions (CSK) of non-treated (NT) and 10 μM Chloroquine (CQ) (24 h) treated HEK-KWGF cells. All three cytoskeleton components were included as well as a ponceau staining, which was used as loading control. Cleaved K_IR2.1-GFP, indicated with an asterisk, is only present after CQ treatment. Fl, full-length K_IR2.1-GFP. (B) Quantified results of the CQ induced induction of K_IR2.1-GFP expression (n = 6). One-way ANOVA was used to calculate significance. (C) Quantified results of cleaved/fl K_IR2.1-GFP ratios in all cell fractions (n = 6). One-way ANOVA was used to calculate significance.