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. 2021 Sep 20;30(2):915–931. doi: 10.1016/j.ymthe.2021.09.017

Figure 3.

Figure 3

Overexpression of circCdyl promotes Ang II-induced AAA formation and increases the levels of inflammatory molecules associated with M1 macrophages

(A) Images showing the characteristics of aortas from Ang II-infused C57BL/6J mice transfected with circCdyl-overexpressing AAV (AAV-circCdyl) or corresponding control AAV (AAV-GFP). (B) Incidence of AAA in Ang II-infused C57BL/6J mice in the two indicated groups. ∗∗p < 0.01; n = 36 per group. (C) Survival curves of Ang II-infused C57BL/6J mice in the two indicated groups. ∗p < 0.05; n = 36 per group. (D) Maximal abdominal aortic outer diameters from Ang II-infused C57BL/6J mice in the two indicated groups. ∗∗p < 0.01; n = 12 per group. (E) EVG staining images and elastin degradation score in the two indicated groups (scale bars, 200 and 50 μm). ∗∗p < 0.01; n = 12 per group. (F) Plasma concentrations of IL-6, MCP1, and TNF-α in the aortas of Ang II-infused C57BL/6J mice in the two indicated groups. ∗∗p < 0.01; n = 5 per group. (G–I) Immunohistochemical staining of iNOS (G) and MMP9 (H) and corresponding densitometric analysis (I) in aortas from Ang II-infused C57BL/6J mice in the two indicated groups (scale bars, 200 and 50 μm). ∗∗p < 0.01; n = 6 per group. (J) CD38, iNOS, and MMP9 levels in the aortas of Ang II-infused C57BL/6J mice in the two indicated groups (western blot) (β-actin as the internal reference). ∗∗p < 0.01; n = 5 per group.