Figure 7.
circCdyl induces M1 macrophage polarization through its interaction with IRF4.
(A) iNOS, MMP9, and CD38 protein levels in peritoneal macrophages transfected with oe-vector, oe-circCdyl, or oe-circCdyl + oe-IRF4 and then stimulated with LPS+IFN-γ (24 h). ∗∗p < 0.01; n = 5 per group. (B) CD206 and Arg1 protein levels in peritoneal macrophages transfected with oe-vector, oe-circCdyl, or oe-circCdyl + oe-IRF4 and then stimulated with IL-4 (24 h). ∗∗p < 0.01; n = 5 per group. (C) iNOS, MMP9, and CD38 protein levels in peritoneal macrophages transfected with si-scramble, si-circCdyl, or si-circCdyl + si-IRF4 and then stimulated with LPS+IFN-γ (24 h). ∗∗p < 0.01; n = 5 per group. (D) CD206 and Arg1 protein levels in peritoneal macrophages transfected with si-scramble, si-circCdyl, or si-circCdyl + si-IRF4 and then stimulated with IL-4 (24 h). ∗∗p < 0.01; n = 5 per group.