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. 2021 Aug 8;30(2):564–578. doi: 10.1016/j.ymthe.2021.08.010

Figure 3.

Figure 3

Recognition of malignant B cells by selected T cell clones

IFN-γ production by T cell clones after overnight stimulation with various target cells. Values and error bars represent mean and standard deviations of technical duplicates. Target cells were positive (+), negative (−), or transduced (Td) with target HLA. Target gene expression was measured by qRT-PCR and is depicted below targets as expression relative to housekeeping genes (HKG set to 1). Allo HLA T cell clones were included as positive controls for HLA expression. (A) FCRL5 HLA-A1-specific T cell clone stimulated with patient-derived CLL samples, in an E:T ratio of 1:20 to correct for cell size and negative control K562 cells in E:T ratio 1:6. T cell clone 6B10.12 could not be tested due to lack of expansion. (B) VPREB3 HLA-A24 clones stimulated with different ALL cell lines and K562 cells in E:T ratio 1:6. (C) BOB1 HLA-B35 clone stimulated with different ALL cell lines, MM cell lines UM9 and U266, and K562 cells in E:T ratio 1:6.