Figure 6.

Antigen-specific killing of B cell malignancies by TCR Td CD8 T cells

Killing by CD8 T cells Td with selected TCRs (in pink), CMV (pp65-NLV-HLA-A2) TCR Td CD8 T cells (in black) as negative control, and allo-HLA-A1, -A24 or -B35 T cell clones (in gray) as positive controls. Target cells were patient-derived HCL, CLL, MCL, and ALL; diffuse large B cell lymphoma cell line TMD8; MM cell line U266; and antigen-negative target HLA Td K562 cells. Target cells endogenously expressed target HLA (HLA^pos) or were Td with target HLA alleles (+HLA). Killing was measured by ⁵¹CR release assay after 6-h co-culture in different E:T ratios. Values and error bars represent mean and standard deviations of technical triplicates. Experiments are representative of two independent experiments. (A) Killing by FCRL5 HLA-A1-specific TCR 6B10.12 Td T cells. (B) Killing by VPREB3 HLA-A24-specific TCR 6A2.18 Td T cells. (C) Killing by BOB1 HLA-B35-specific TCR 1C5.6 Td T cells.