Figure 5.
Changes in cancer stromal cells after treatment with ClyA-expressing bacteria
All samples (n = 6 mice per group) were collected from AsPC-1 subcutaneous cancer model mice at 5 days after bacterial infection (3.0 × 107 CFU; 48 h after ClyA induction). (A) Expression of stromal cell markers was analyzed by western blotting; the signal was normalized to that of β-actin (n = 6). (B) Expression of representative markers was confirmed with immunofluorescence staining. NG2, neural/glial antigen 2; PDGFRβ, platelet-derived growth factor receptor β; CD31, cluster of differentiation 31. (C) Immune cell infiltration after treatment with SLlux/ClyA (n = 6). Neu, neutrophil marker; F4/80, macrophage marker. Scale bars, 50 μm.